Development of an electrochemical biosensor for bacteria detection coupling immuno-capture with magnetic particles and amperometry at flow-channel microband electrodes.
Current technology is insufficient for rapid on-site identification of the causative agents for waterborne diseases and existing time-consuming detection results in delayed management decisions. Fast, reliable and low-cost methods for the screening of pathogens are paramount in fields such as the environment, food industry, healthcare and defense. With the constant progress of scientific knowledge, a fast diversification of detection techniques is occurring, brought about by the appearance of imaginative new concepts within the scientific community. Biosensors are a perfect example of the combination of multidisciplinary knowledge. They encompass many fundamental, technological and scientific advances in biology, chemistry and physics.
Here, we describe a recently developed electrochemical biosensor for the detection of bacteria cells in aqueous samples. The technology used for this detection combines immuno-magnetic capture and amperometric detection in a one-step sandwich format, and in a microfluidic environment. The whole assay could be completed in 1 h and the experiments performed with Escherichia coli evidenced a linear response for concentrations ranging 102–108 cell ml−1.