Streptomyces coelicolor

Event Date: 
Wednesday, May 28, 2014 - 18:00 - 18:15
Institution: 
UNSW
Title: 

Regulation of Secondary Metabolites Production in Streptomyces coelicolor

Abstract: 

Streptomyces coelicolor produces a wide array of secondary metabolites that include antibiotics, actinorhodin (Act) and undecylprodigiosin (Red). When analysed for the growth of S. coelicolor on blood containing medium, we found that a functional haemolysin was critical for its growth. Gene knock-out and complementation analysis revealed the expression haemolysin was critical for growth, sporulation and antibiotics production. Total proteome comparison of wild-type and haemolysin deletion mutant indicated a wide range of carbon metabolizing enzymes to be down-regulated. Further analysis of selected targets that could encode phosphomannose isomerase and alpha-mannosidase revealed these sugar processing enzymes to play a critical role on growth, sporulation and antibiotics production in S. coelicolor.

Event Date: 
Wednesday, April 30, 2014 - 18:15 - 18:30
Institution: 
CSIRO
Title: 

Genetic diversity of Group I Clostridium botulinum and Clostridium sporogenes

Abstract: 

Whilst classified as a single bacterial species, Clostridium botulinum comprises a phylogenetically and physiologically diverse collection of organisms. Members of this species are linked together based solely on the production of botulinum neurotoxin (BoNT); amongst most lethal natural toxin produced. Isolates that do not produce BoNT are taxonomically considered a separate species, such as Clostridium sporogenes. Given the species delineation is based solely on an unstable phenetic trait presents increasing challenges in a post-genomic era, particularly with increasing evidence pointing towards the lateral acquisition of BoNT production in many strains. Here, the pan-genome of Group I C. botulinum and C. sporogenes is presented, describing the genetic diversity of these species, highlighting the incongruent taxonomy of these organisms and presenting insights into the acquisition of BoNT within this group.

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