Regulation of Secondary Metabolites Production in Streptomyces coelicolor
Streptomyces coelicolor produces a wide array of secondary metabolites that include antibiotics, actinorhodin (Act) and undecylprodigiosin (Red). When analysed for the growth of S. coelicolor on blood containing medium, we found that a functional haemolysin was critical for its growth. Gene knock-out and complementation analysis revealed the expression haemolysin was critical for growth, sporulation and antibiotics production. Total proteome comparison of wild-type and haemolysin deletion mutant indicated a wide range of carbon metabolizing enzymes to be down-regulated. Further analysis of selected targets that could encode phosphomannose isomerase and alpha-mannosidase revealed these sugar processing enzymes to play a critical role on growth, sporulation and antibiotics production in S. coelicolor.