Kerensa McElroy (UNSW) started us off immersing the audience in deep sequencing in order to understand pathogen evolution in biofilms. Two model pathogens, Phaeobacter gallaeciensis and Pseudomonas aeruginosa, were used to grow biofilms under conditions that select for reproducible phenotypic diversification. Variations in the genetic structure were revealed addressing different stages of biofilm development. Kerensa could describe genetic variation accurately and comprehensively within evolving populations using her established approach in genome-wide deep sequencing.
Pushing temporal and spatial resolution limits- Light and Optical Microscopy in 2012.
The last 10 years has seen major changes in the way light and fluorescence microscopy is used as a tool by researchers from most scientific disciplines. These advances broach specimen preparation, hardware and the analysis of the images. In particular microscopes have become increasingly more sophisticated; they image faster, longer, at lower light levels, and at greater spatial resolution than ever anticipated. The scope of this talk is to familiarize the audience with some of the latest techniques in light and optical microscopy including; live cell imaging, confocal microscopy and the superresolution techniques such as STED, SIM and PALM. The focus will be on understanding what the temporal and spatial resolution that in practice can be obtained and whether a technique can be used to obtain qualitative AND quantitative data for your experiment. Lastly, the main challenges facing researchers using light and optical microscopy will be presented.